Fig. 4.

Selections with an SMPL. (A) Immobilized metal affinity chromatography microtiter plates were used as the capture target for three His-tagged epitope variants. (B) SMPL display. A BP variant displaying a FLAG epitope (Mtd FLAG₂-367) or BP (wild type) were incubated in anti-FLAG-coated microtiter wells or control wells lacking the antibody. Error bars represent standard error (n = 3). (C) Selections for HIV gp41 binding. Target (gp41)-coated microtiter plates were used to capture mutants displaying a library based upon the sequences (AAC)₄, (AAC)₈ or wild-type BP (no insert) during four rounds of selections. Bound BP or mutants were eluted and amplified as in (A). Strong enrichment was observed for the SMPLs with the peptides inserted into the Mtd, and no enrichment resulted from the wild-type Mtd SMPL.