Table I.
Epitope variants and insert positions tested
| Mtd insert position | Description | Sequence |
|---|---|---|
| TR VARIANTS | ||
| 342, 344, 346, 347, 348,349, 351, 367, 368 | His6 | HHHHHH |
| 342, 344, 346, 347, 348, 349, 351,367, 368 | His8 | GGSHHHHHHHHSGG |
| 348 | hdd-His10 | KFLIVKSGPAGHHHHHHHHHHGGAPGEFLIVES |
| 348 | His6LOOP | GGSGHHHHHHNGPHHHHHHGSGG |
| 348 | 2XHis | HHHHHHSNSGSRAVNWNNTHHHHHH |
| 342, 344, 346, 347, 348, 349, 351, 367, 368 | FLAG | LGDYKDDDDK |
| 367 | FLAG2 | GGSDYKDDDDKDYKDDDDKSGG |
| 348 | hdd-FLAG | KFLIVKSGPAGDYKDDDDKGGAPGEFLIVES |
| 367 | c-myc | GGSEQKLISEEDLSGG |
| 348, 367 | Lys8 | GGSKKKKKKKKSGG |
| 348,367 | Asn4 | GGSNNNNSGG |
| 348, 367 | Asn8 | GGSNNNNNNNNSGG |
| VR VARIANTS | ||
| 348, 368 | His6LOOP | GGSGHHHHHHNGPHHHHHHGSGG |
| 367 | His6 | HHHHHH |
| 367 | c-myc | GGSEQKLISEEDLSGG |
| 367 | Asn4 | GGSNNNNSGG |
| 367 | His8 | GGSHHHHHHHHSGG |
| 367 | FLAG2 | GGSDYKDDDDKDYKDDDDKSGG |
| 367 | AG | AG |
| TR/VR VARIANTS | ||
| 347 | His6 | HHHHHH |
| 348 | 2XHis/His6LOOP | HHHHHHSNSGSRAVNWNNTHHHHHH/GGSGHHHHHHNGPHHHHHHGSGG |
Results with underlined constructs are described in the text.
Residues not underlined failed viability or variability assays.
hdd, homodimerization domain (Marks et al., 2004).