Fig 4.

REF/Aly is sufficient to increase PAN RNA abundance. (A) Diagram of the PAN RNA expression constructs used in the tethering experiments. PAN RNA is driven by its own promoter and has its own polyadenylation signals (black rectangles). Δ, absence of the ENE; b.s., MS2 binding sites. (B) Representative Northern blots from tethering experiments. The presence or absence of MS2 binding sites and the amounts of cotransfected MS2 construct are shown above the panels. The probe and PAN RNA expression construct are indicated on the left. Panels have different exposures, to best display the given signal, but relative quantification is shown in panel C. The loading control detects RNA from a cotransfected mgU2-19/30 plasmid that expresses a previously described murine scaRNA (11, 75). (C) Quantification of the data from multiple REF-MS2 tethering assays. The data are mean values relative to the matched no-REF-MS2 or no-MS2 control. The error bars show standard deviations (n = 3).