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REF/Aly tethering does not promote PAN RNA export. In situ hybridization of PANΔENE RNA containing MS2 binding sites (top and middle) or lacking MS2 binding sites (bottom) is shown. MS2 and REF-MS2 were cotransfected as indicated. (Left) PAN RNA probe; (middle) DAPI-stained nuclei; (right) merged signal. The PAN RNA plus REF-MS2 panels are shown at a lower exposure to avoid saturation of the signal.
