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Ras/MAPK signaling from mice with Ctr1-deficient hearts recapitulates cell culture observations. Heart lysates from two Ctr1 wild-type (Ctr1flox/flox) animals were analyzed by immunoblotting for Mek1, Mek2, phospho-Mek1/1, Erk, phospho-Erk, CCS, and tubulin as a loading control. C indicates an individual Ctr1flox/flox mouse, and M indicates an individual Ctr1hrt/hrt mouse analyzed.
