Fig 5.

Interactions between MecA and ComX, ClpC, or ClpE evaluated by B2H assays. (A to C) Matrices of bacterial two-hybrid interactions between MecA and ComX (A), MecA and ClpC (B), and MecA and ClpE (C) on MacConkey indicator plates supplemented with 1% maltose. Plates were incubated at 30°C for 36 h. Red, green, and dotted black rectangles indicate MecA-ComX, MecA-ClpC, and MecA-ClpE interaction assays, respectively. Dark blue, light blue, and magenta rectangles indicate MecA, ClpC, and ClpE self-interaction assays, respectively. Controls are surrounded by a black line; 25 and 18 correspond to the empty vectors pKT25 and pUT18, respectively. (D) Summary of interactions detected by B2H assays suggesting a ternary complex, ClpC-MecA-ComX. The arrow color code refers to the interaction assays described above for panels A, B, and C.