Table 2.
Maximum luciferase activities of S. thermophilus strains
| Strain | Promoter | Relevant feature(s)b | Mean max luciferase activity ± SEMa |
|
|---|---|---|---|---|
| THBG conditions (RLU/OD600 × 104) | CDML conditions (RLU/OD600 × 106) | |||
| LMD-9 derivatives | ||||
| CB007 | PcomGA | Wild type | 3.5 ± 1.0 | 14.6 ± 0.5 |
| CB0071 | PcomGA | MecA− (Cmr) | 353.6 ± 32.1 | 14.5 ± 0.3 |
| CB0072 | PcomGA | MecA− | 389.0 ± 77.3 | 16.7 ± 0.5 |
| CB0078 | PcomGA | ComX− | 1.6 ± 0.5 | NT |
| AW007 | PcomGA | MecA− ComX− | 1.7 ± 0.4 | NT |
| AW002 | PcomGA | ClpC− | 280.6 ± 26.1 | NT |
| AW004 | PcomGA | ClpE− | 4.2 ± 1.3 | NT |
| AW005 | PcomGA | MecA− ClpC− | 300.0 ± 33.5 | NT |
| AW006 | PcomGA | MecA− ClpE− | 318.0 ± 49.7 | NT |
| CB0071(pXLΔ1) | PcomGA | MecA−, empty vector | 337.1 ± 8.5 | NT |
| CB0071(pGICB005) | PcomGA | MecA−, complementation vector | 4.1 ± 0.6 | NT |
| CB001 | PcomX | Wild type | 6.3 ± 0.6 | 33.1 ± 2.0 |
| CB0011 | PcomX | MecA− (Cmr) | 6.7 ± 0.7 | 28.6 ± 5.9 |
| CB0012 | PcomX | MecA− | 8.0 ± 0.5 | 16.2 ± 2.7 |
| LMG18311 derivatives | ||||
| CB006 | PcomGA | Wild type | 0.2 ± 0.02 | 0.2 ± 0.04 |
| CB0061 | PcomGA | MecA− (Cmr) | 39.2 ± 1.6 | 5.3 ± 0.4 |
a
Mean values from triplicate experiments ± SEM. NT, not tested.
b
Cmr, chloramphenicol resistance resulting from the replacement of mecA with cat.