Table 1.
Xenorhabdus strains used in this study
| Strain type | Strain | Parent strain | Relevant characteristic(s) | Source or reference |
|---|---|---|---|---|
| Insertionlessa | HGB007 | NAb | Sequenced ATCC 19061 X. nematophila wild type | ATCC |
| HGB777 | HGB007 | Δ(nilA-nilC)7::Km (ΔSR1) | 15 | |
| HGB1251 | HGB777 | Δ(nilA-nilC)7::Km (ΔSR1) ΔnilR16::Sm | This study | |
| HGB1430 | HGB777 | Δ(nilA-nilC)7::Km lacZp-GFP (from pJMC001) | This study | |
| HGB1429 | HGB1251 | Δ(nilA-nilC)7::Km ΔnilR16::Sm lacZp-GFP (from pJMC001) | This study | |
| Non-GFP-expressing nilB FLAG insertion mutants | HGB778 | HGB777 | SR1 | 15 |
| HGB1256 | HGB777 | SR1/nilB26-FLAG aa026 (nilB-FLAG26) | This study | |
| HGB1200 | HGB1251 | SR1/nilB26-FLAG aa026 (nilB-FLAG26) | This study | |
| HGB1805 | HGB1251 | SR1/nilB27-FLAG aa068 | This study | |
| HGB1201 | HGB1251 | SR1/nilB28-FLAG aa085 | This study | |
| HGB1806 | HGB1251 | SR1/nilB29-FLAG aa102 | This study | |
| HGB1807 | HGB1251 | SR1/nilB30-FLAG aa121 | This study | |
| HGB1203 | HGB1251 | SR1/nilB31-FLAG aa137 | This study | |
| HGB1254 | HGB1251 | SR1/nilB32-FLAG aa186 | This study | |
| HGB1204 | HGB1251 | SR1/nilB33-FLAG aa233 | This study | |
| HGB1253 | HGB1251 | SR1/nilB34-FLAG aa273 | This study | |
| HGB1206 | HGB1251 | SR1/nilB35-FLAG aa322 | This study | |
| HGB1260 | HGB1251 | SR1/nilB36-FLAG aa357 | This study | |
| HGB1808 | HGB1251 | SR1/nilB37-FLAG aa379 | This study | |
| HGB1207 | HGB1251 | SR1/nilB38-FLAG aa399 | This study | |
| HGB1809 | HGB1251 | SR1/nilB39-FLAG aa421 | This study | |
| HGB1208 | HGB1251 | SR1/nilB40-FLAG aa451 | This study | |
| HGB1324 | HGB1251 | SR1/nilB41-FLAG aa466 | This study | |
| HGB1255 | HGB1251 | SR1 | This study | |
| GFP-expressing nilB FLAG insertion mutants | HGB1502 | HGB1430 | SR1/nilB34-FLAG aa270 | This study |
| HGB1495 | HGB1429 | Empty | This study | |
| HGB1496 | HGB1429 | SR1 (wild type nilB without FLAG) | This study | |
| HGB1484 | HGB1429 | SR1/nilB26-FLAG aa026 (nilB-FLAG26) | This study | |
| HGB1487 | HGB1429 | SR1/nilB32-FLAG aa186 | This study | |
| HGB1489 | HGB1429 | SR1/nilB34-FLAG aa273 | This study | |
| HGB1490 | HGB1429 | SR1/nilB35-FLAG aa322 | This study | |
| HGB1492 | HGB1429 | SR1/nilB38-FLAG aa399 | This study | |
| GFP-expressing nilB-FLAG26 deletion mutants | HGB1460 | HGB1429 | SR1/nilB42-Δ026–042 | This study |
| HGB1461 | HGB1429 | SR1/nilB43-Δ043–059 | This study | |
| HGB1462 | HGB1429 | SR1/nilB44-Δ026–059 | This study | |
| HGB1463 | HGB1429 | SR1/nilB45-Δ076–091 | This study | |
| HGB1464 | HGB1429 | SR1/nilB46-Δ092–108 | This study | |
| HGB1465 | HGB1429 | SR1/nilB47-Δ026–108 | This study | |
| HGB1510 | HGB1429 | SR1/nilB48-Δ109–156 | This study | |
| HGB1511 | HGB1429 | SR1/nilB49-Δ026–156 | This study | |
| HGB1466 | HGB1429 | SR1/nilB50-Δ266–279 | This study | |
| HGB1467 | HGB1429 | SR1/nilB51-Δ292–298 | This study | |
| HGB1468 | HGB1429 | SR1/nilB52-Δ314–320 | This study | |
| HGB1469 | HGB1429 | SR1/nilB53-Δ352–357 | This study | |
| HGB1470 | HGB1429 | SR1/nilB54-Δ389-410 | This study |
a
All strains except HGB007, HGB777, HGB1251, HGB1430, and HGB1429 had att Tn7 insertions.
b
NA, not applicable.