Table 3.
Fold change in two independent experiments between bacterial load and nematode colonization frequency of nilB-FLAG insertion mutants
| FLAG insertion | Expt 1 |
Expt 2 |
||||
|---|---|---|---|---|---|---|
| Relative bacterial load (%)a | Relative frequency (%)b | Fold differencec | Relative bacterial load (%) | Relative frequency (%) | Fold difference | |
| aa 26 | 55.882 | 89.490 | 1.60 | 119.156 | 102.007 | 1.17 |
| aa 186 | 52.574 | 52.352 | 1.00 | 61.039 | 49.763 | 1.23 |
| aa 273 | 106.985 | 96.734 | 1.11 | 99.675 | 91.455 | 1.09 |
| aa 322 | 32.279 | 25.762 | 1.25 | 60.390 | 38.542 | 1.57 |
| aa 399 | 1.397 | 0.960 | 1.46 | 4.789 | 4.721 | 1.01 |
| eTn7 | 0.042 | 0.015 | 2.86 | 0.009 | 0.009 | 1.00 |
| None | 100.000 | 100.000 | 1.00 | 100.000 | 100.000 | 1.00 |
Bacterial load was assessed by sonication of 10,000 nematodes and dilution plating to determine bacterial counts. Data are presented as percentages of the value for cells expressing wild-type nilB with no FLAG insertion.
Nematode colonization frequency was assessed by epifluorescence microscopy of nematodes grown on GFP-expressing bacteria. Data are presented as percentages of the value for cells expressing wild-type nilB with no FLAG insertion.
Fold difference represents the ratio of relative colonization frequency to relative bacterial load. A ratio of 1 indicates that the colonization defect of the strain is explained by a reduced frequency of colonization (e.g., initiation). A ratio of greater than 1 indicates that a defect in outgrowth contributes to observed defects in bacterial load.