Fig 2.

Primer extension on total RNA isolated from E. coli cells carrying plasmid pMV158. The primer (Table 1, mobM-PE) annealed to the transcripts in the region corresponding to coordinates 3725 to 3748 of pMV158. The size expected for the cDNAs if transcription initiation occurs at coordinate 3609 (Pmob1) or 3643 (Pmob2) is indicated below the gel. Primer extension products were resolved on denaturing gels (7 M urea–8% polyacrylamide). As DNA size markers, dideoxy-mediated chain termination sequencing reactions using pMV158 DNA and the mobM-PE primer were run in the same gel (lanes A, G, T, and C). The sizes of the cDNA extension products (lane P) are indicated on the right in nucleotides.