Table 1.
Oligonucleotides used in this work
| Primer name | Sequence (5′ to 3′)a | Coordinates (nt)b |
|---|---|---|
| DraF | GGTGGAGATTTTTTGAGTG | 3371–3389 |
| DraR | CACGTTCATTATGCTTTAAAGCTCCTCCC | 3807–3779 |
| ssoUF | GGGATCAACTTTGGGAGAGA | 3121–3140 |
| ssoUR | GCGTCTCAAAAACACGTTCA | 3819–3800 |
| ssoAR | TCACAACGCTCACCTCCA | 5495–5478 |
| P−116 | TTATGGTTTTGGTCGGCACT | 3527–3546 |
| P+46 | CACGAGCCGACACAGTCTATT | 3688–3668 |
| ORI | GGCACTGCCGAATTCCTCGCAGAGC | 3541–3565 |
| IR2 | ACACTTTATGAATTCAAAGTATAGTGT | 3568–3594 |
| MR | CAGACGAGCCGCTGCAGTCTATTGCT | 3690–3665 |
| mobM-PE | GCAACCATGTAACTCATAGATTTC | 3748–3725 |
| lacZ-PE | GTGATTTTTTTCTCCATTTTAGC | |
| tetA-PE | GGGGTATGTTGGGTTTCACGTCTG | |
| UC-50 | TTGTGAGCGGATAACAATTTC |
a
Restriction sites are in bold face, and base changes that generate restriction sites are underlined.
b
Coordinates are given with respect to pMV158 sequence (accession number NC_010096).