Fig 1.

HERV-Fc1 gag DNA copy number calculations in the control group for the two genders. Shown are SYBR green absolute Q-PCR assay analyses of HERV-Fc1-positive healthy controls; HERV-Fc1 gag gene copy numbers are calculated per human genome equivalent (30 subjects for each gender). The data are expressed as median copy numbers/reaction plus standard deviation (SD), quantified on the basis of an external DNA calibration curve obtained from serial dilutions of plasmids containing the amplicon of interest, as described in Materials and Methods. The P value was calculated using the nonparametric Mann-Whitney U test.