Table 2.

Comparison of C_T values for standard curves and intra-assay analyses

Reference standard (copies/reaction, 2 μl)	Mean CT valuea	SD	CV
DNA dilutions
2.0E8	8.56	0.14	1.64
2.0E7	12.49	0.02	0.16
2.0E6	16.36	0.29	1.7
2.0E5	20.09	0.05	0.24
2.0E4	23.87	0.02	0.08
2.0E3	27.62	0.5	1.8
2.0E2	31.79	0.17	0.53
2.0E1	34.61	0.06	0.17
RNA dilutions
5.52E8	13.56	0.02	0.15
5.52E7	17.03	0.05	0.3
5.52E6	22.61	0.7	3.1
5.52E5	26.49	0.51	1.9
5.52E4	31.43	0.2	0.6
5.52E3	34.46	0.31	0.89
5.52E2	39.78	0.4	1
5.52E1	ND

^aFor each sample, the C_T value is an average of results from four replicates. ND, not determined.