Table 3.

Interassay analysis of mean C_T values

Reference standard (copies/reaction, 2 μl)	Mean CT valuea	SD	CV
DNA dilutions
2.0E4	23.95	0.1	0.4
2.0E3	27.23	0.3	1.1
RNA dilutions
5.52E6	22.81	0.2	0.8
5.52E5	26.79	0.31	1.1

^aFor each sample, the C_T value is an average of three different experiments performed in duplicate.