Fig 7.
Specific infection of cells overexpressing αvβ5 integrins with the RGD-4C-containing H-1PV-H373R mutant. (A) CS-1 parental cells and derivative CS-1/β3 and CS-1/β5 cell lines were assessed for αvβ3 and αvβ5 protein expression by flow-cytometric analysis as described in Materials and Methods. (B) CS-1, CS-1/β3, and CS-1/β5 cell lines were grown in the absence (untreated) or presence of the wild type (H-1PV) or the RGD-4C-containing H-1PV mutant (H-1RGD) for 24 or 48 h before they were processed for Western blot analysis and the detection of NS1, VP1, and VP2 viral protein levels. β-Tubulin was used as a loading control.