Fig 4.

LMP1-DNs impair NF-κB survival signaling but not the c-Jun N-terminal kinase (JNK) signaling pathway in EBV-transformed lymphocyte (NC5) lines. LMP1-DN expression was induced by doxycycline for 0, 24, and 48 h. Then the cells were lysed as described for Fig. 3. Shown are the effects of LMP1-DNs on the NF-κB (A) and JNK (B) pathways. Total-cell lysates were analyzed by Western blotting using antibodies to phosphorylated IκB, phosphorylated JNK, JNK, and β-actin. The intensities of bands on the Western blot were quantified, and the results obtained for cells expressing LMP1-DNs or GFP were compared to those for the control (without Dox) after normalization to actin expression (A) or JNK expression (B).