Fig 1.

Screening of molecules that reactivate HIV from latency in an in vitro cellular model. (A) Chemical structures of active compounds identified in a screening for HIV activation. Upon screening a library of 6,000 small molecules using a cell line containing a latent HIV-based retroviral vector (J-Lat clone A2), eight compounds were identified that reproducibly lead to an induction of HIV expression. Six of these compounds were grouped into two families according to their structural similarity, classes 1 and 2. Hits 4 and 1 are representative of these classes 1 and 2, respectively. (B) Dose response of hits 1 and 4. Cells from the J-Lat heterogeneous population were incubated with increasing concentrations of drug for 24 h and analyzed by flow cytometry. HIV-GFP reactivation is reported as the percentage of GFP-expressing cells (%GFP). Cell viability was measured by flow cytometry gating on the live population at the forward scatter (FSC) and side scatter (SSC) plot. An example is shown in panel C.