Fig 1.

Examination of the relative abundance of the major ND10 factors PML, Sp100, and hDaxx during infection with HVS and MHV-68, respectively. (A to C) Immunoblots of primary HFFs infected with MHV-68/GFP or HVS-GFP at a high MOI. Cell lysates were harvested at 24 h postinfection and subjected to SDS-PAGE. The blots were probed with antibodies against the three major ND10 constituents, PML (A), Sp100 (B), and hDaxx (C), as indicated on the right of the panels. Detection of beta-actin was included as an internal loading control. Molecular masses are indicated to the left of each panel. (D) Immunofluorescence analysis of HVS-GFP-infected HFF cells. At 24 h postinfection, cells were fixed and stained for the ND10 factors PML, hDaxx, and Sp100. HVS-positive cells were identified by GFP expression. Cell nuclei were visualized by costaining with DAPI.