Fig 2.

Sp100 downregulation of nonhuman primate origin by HVS. (A to C) Fibroblast cells (Fibs) derived from rhesus macaques (Macaca mulatta) were either mock infected (A and B) or infected with HVS-GFP (C). Twenty-four hours later, cells were fixed and stained for the major ND10 components PML (anti-PML rabbit antiserum [PML-r] generated by Peter Hemmerich, Jena, Germany,), hDaxx (Daxx-m; MCA2143), and Sp100 (ProteinTech Group). No signals were obtained with the monoclonal antibody PG-M3 against PML (A, panel c, PML-m) and the anti-Sp100 rabbit antibody (Sp100-r; generated by Peter Hemmerich, Jena, Germany) (A, panel f). HVS-positive cells were identified by GFP expression. Cell nuclei were visualized by costaining with DAPI.