Fig 3.

HVS-induced Sp100 degradation occurs by the proteasome and is associated with a virion component. (A) Immunoblots of HFFs infected with wt HVS-GFP at a high MOI that were either untreated or treated with 5 μM of the proteasome inhibitor MG132 or with 150 μg/ml of cycloheximide (CHX). The cells were harvested at 8 h postinfection (8 hpi), and the relative protein levels of Sp100, PML, and GFP were analyzed. Beta-actin served as a loading control. (B) HFF cells were mock infected or infected with HVS-GFP for 10 h. Some cells were simultaneously treated with MG132 or CHX at the time of virus inoculation, as indicated at the left of the respective panels. The cells were stained with antibodies directed against PML and Sp100. Cell nuclei were detected by DAPI staining.