Fig 9.

Analysis of HVS ΔORF3-GFP replication in the absence of the three major ND10 factors PML, hDaxx, and Sp100. Retrovirally transduced HFFs expressing the respective siRNAs, as indicated (vector, siC, siPML2, siDaxx1, and siSp100), were infected with recombinant HVS ΔORF3-GFP. (A) GFP fluorescence 6 days after infection of the indicated HFFs; (B) direct quantification of the number of GFP-positive cells at day 6 postinfection; (C) Western blot analyses of HVS ΔORF3-GFP gene expression in the retrovirally transduced HFFs (vector, siC, siPML2, siDaxx1, and siSp100) using an antibody directed against GFP. The detection of beta-actin served as a loading control.