The C-terminal 270 amino acids of HSV1 pUL36 are dispensable for secondary envelopment and virion assembly. Vero cells were infected at an MOI of 10 PFU/cell with pHSV1(17+)blueLox (wt; A and B), the mutant HSV1(17+)blueLox-ΔUL36 (ΔUL36; C and D), or HSV1(17+)blueLox-UL36codon2211stop (2211; E and F), -UL36codon2894stop (2894; G and H), or -UL36codon2998stop (2998; I and J) and prepared for electron microscopy after 14 h or 12 h (E and F, respectively). Bars = 200 nm (A, C, E, G, and I) and 50 nm (B, D, F, H, and J).