Fig 12.

The C-terminal 167 amino acid residues of HSV1 pUL36 are required to maintain capsid association during cell entry. Vero cells were infected at an MOI of 0.2 PFU/cell with pHSV1(17⁺)blueLox (wt; a to d) or mutant HSV1(17⁺)blueLox-UL36codon2894stop (2894; e to h) or -UL36codon2998stop (2998; i to l), transcomplemented with pUL36 by amplification on Vero-HS30 cells, and fixed at 15 h p.i. with 3% paraformaldehyde, followed by TX-100 permeabilization. The specimens were labeled with antibodies directed against VP5 (red and b, f, and j; MAb 5C10) or pUL36 (green and c, g, and k; middle, pAb 147) and analyzed by confocal fluorescence microscopy. The insets (white squares) are shown at higher magnification in the horizontally neighboring panels. The location of the cell borders was estimated based on staining with TRITC-phalloidin for filamentous actin (blue in a, e, and i; continuous white lines), and the position of the cell nuclei was determined by differential interference contrast (dashed white lines).