Fig 6.

NiV infection in cathepsin-knockout MEFs. MEFs derived from wild-type (wt), cathepsin L-knockout (L^−/−), cathepsin B-knockout (B^−/−), or double-knockout (L^−/−/B^−/−) mice were infected with NiV. (A) At 48 h p.i., NiV infection was visualized by immunostaining with a polyclonal anti-NiV serum and rhodamine-conjugated secondary antibodies, and nuclei were counterstained with DAPI. ϕ, average. (B) To quantify virus release, supernatants were collected at 48 h p.i., and virus titers were determined by the TCID₅₀ method. One representative example from three independent experiments is shown. Virus titers in wild-type MEFs were set at 100%.