Fig 3.

W956IC infection induces PKR and eIF2α phosphorylation. (A) Western blot analysis of BHK cells infected with WNV Eg101 or W956IC at an MOI of 1. Lysates were collected in radioimmunoprecipitation assay (RIPA) buffer at the indicated times (hours) after infection and analyzed using antibodies to phosphorylated PKR (Thr541), PKR, phosphorylated eIF2α (Ser51), eIF2α, and actin. M, mock. (B) BHK cells were infected with WNV Eg101 or W956IC at an MOI of 1, fixed at 24 h after infection, and analyzed by confocal microscopy. Anti-G3BP antibody (green) was used to detect SGs, and anti-p-eIF2α antibody (white) was used to detect p-eIF2α. More than 95% of the cells were infected.