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EV71 inhibited phosphorylation of STAT1/STAT2. RD cells (A and C) or HeLa cells (B and D) were infected with EV71 at an MOI of 1 or 10 for 9 h, and cells were left untreated (−) or treated (+) with IFN-α2b (A and B) or IFN-β (C and D) for another 30 min. Western blotting was performed by detecting STAT1, STAT2, phosphorylated STAT1 (p-STAT1), phosphorylated STAT2 (p-STAT2), and viral structural protein VP1 with relative antibodies. GAPDH was also detected as a loading control.
