Fig 2.

Coimmunoprecipitation of A26 protein with multiple components of the viral EFC in virus-infected cells. (A) Coimmunoprecipitation of A26 with EFC components in virus-infected cells. HeLa cells were either mock infected or infected with WR or WR-Flag-A26 virus at an MOI of 5 PFU/cell, harvested at 24 h p.i. for immunoprecipitation (IP) with anti-Flag-agarose, and analyzed by immunoblotting with various antibodies, shown at the right side of the gel. (B) Coimmunoprecipitation of G9 protein with A26 protein in virus-infected cells. HeLa cells were either mock infected or infected with wild-type WR or vA28i virus at an MOI of 5 PFU/cell, incubated in medium with or without 100 μM IPTG, harvested at 24 h p.i. for immunoprecipitation with anti-G9 (1:50) antibody, and analyzed as described for panel A. Input represents 1% of total cell lysates.