Fig 4.

A26 interacts individually with A16 and G9 but not with G3 and L5 proteins in vitro. (A) GST-A26 pulled down A16 and G9 individually. HEK293T cells were transfected with plasmids expressing HA-tagged A16, Myc-tagged G9, or both and harvested 24 h later. The lysates were subjected to GST pulldown analyses using 30 μg of recombinant GST and GST-A26 proteins as described in Materials and Methods and analyzed by immunoblotting with anti-HA (A16) (1:1,000) and anti-Myc (G9) (1:1,000) antibodies. (B) GST-A26 did not pull down G3 and L5. HEK293T cells were transfected with plasmids expressing vaccinia G3 and L5 proteins and harvested 24 h later, and the lysates were subjected to GST pulldown analyses as described above using anti-G3 (1:1,000) and anti-L5 (1:1,000) antibodies. Input represents 1% of total cell lysates.