Fig 6.

A26 does not interact with EFC component proteins H2, G3, and L5 in transfected HEK293T cells. HEK293T cells were transiently transfected with individual plasmids expressing A26-V5 along with A16 and G9 (A) or G3 and L5 (B) or H2 (C) protein, harvested for coimmunoprecipitations with anti-V5 agarose beads, and analyzed by immunoblotting with anti-V5 (1:4,000), anti-A16 (1:1,000), anti-G9 (1:5,000), anti-G3 (1:1,000), anti-L5 (1:1,000), and anti-H2 (1:1,000) antibodies. Input represents 1% of total cell lysates.