Table 2.
Detection of VZV
| Sampling day | Amt of virus detected by PCR (no. of VZV copies/μl)a |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Group 1 |
Group 2 |
Controls |
||||||||
| C88-117M | C87-009M | C91-080M | C04-019M | C89-100M | C95-073Mb | C91-078M | C04-015M | C87-103M | C93-093M | |
| Day 4 | 708 | 1179 | 118 | 7 | 59 | 0.2 | 48 | 16 | − | − |
| Day 10 | 0.34 | − | 0.45 | 0.23 | − | 0.24 | − | 0.05 | − | − |
| Day 21 | 0.38 | − | 0.003 | − | − | − | − | 0.02 | − | − |
| Day 28 | 0.02 | 0.004 | 0.3 | − | − | − | − | 0.004 | − | − |
VZV DNA from BAL samples was amplified via quantitative PCR at the indicated days after initial inoculation. A shell vial culture-based assay was used to detect replicating VZV at day 4 postinoculation. Standards provided with kit were run in duplicate, fluorescent values were averaged, and the standard curve generated was used to determine copy number of unknown test specimens. The dynamic range of the assay was 10 to 10,000 copies/μl; values of <10 copies/μl fall below the validated limit of detection of the assay. All specimens were run containing internal controls in order to identify inhibitory factors in the PCR, and no PCR inhibition was seen with the specimens. For all animals except animal C91-080M, the results of shell vial and DFA assays were negative.
Animal C95-073M coughed immediately after receiving the initial inoculation and thus did not receive the full dose.