Fig 3.

The C-termimal dsRNA binding domain of US11 is responsible for inhibiting SeV-mediated IFN-β promoter activity. (A) Schematic representation of US11 functional domains and deletions. (B) Luciferase assays in HEK 293T cells were performed as for Fig. 1A to measure the activation of the IFN-β promoter following SeV infection in the presence of full-length and deletion mutants of US11, including US11-EYFP, US11(1--83)-EYFP, US11(84-152)-EYFP, US11(84-125)-EYFP, and US11(126-152)-EYFP. Data are expressed as relative luciferase activities with standard deviations for three independent experiments performed in duplicate. (C) The expression of these US11 deletion mutants was verified by Western blotting using rabbit anti-YFP polyclonal antibody.