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. 2012 Apr;86(7):3528–3540. doi: 10.1128/JVI.06713-11

Fig 5.

Fig 5

The carboxyl terminus of US11 interacts with the carboxyl termini of RIG-I and MDA-5. (A and B) HEK293T cells were cotransfected with plasmid pEF-Flag-RIG-IC (A) containing the carboxyl-terminal domain (aa 218 to 925) or pEF-Flag-RIG-IN (B) containing the amino-terminal domain (aa 1 to 229) and with plasmid US11-HA. (C and D) HEK293T cells were cotransfected with plasmid pEF-Flag-MDA-5H (C) containing the carboxyl-terminal helicase domain (aa 287 to 1025) or pEF-Flag-MDA-5C (D) containing the amino-terminal CARD domain (aa 1 to 287) and with plasmid US11-HA. Immunoprecipitation and Western blot analysis were performed as described for Fig. 5A. (E and F) HEK293T cells were cotransfected with plasmid pEF-Flag-RIG-IC (E) or pEF-Flag-MDA-5H (F) and with plasmid US11(84-152)-EYFP or pEYFP-N1, respectively. At 24 h after transfection, immunoprecipitation with anti-Flag MAb or nonspecific mouse monoclonal antibody (IgG2b) and Western blot analysis were performed as described for Fig. 5A.