Fig 10.

Investigation of 4E10 and Z13e1 binding to E168K+N189A WT VLPs by a BN-PAGE Western blot. The nature of the ∼200-kDa species induced by 4E10 and Z13e1 binding to E168K+N189A WT trimer VLPs (indicated by an asterisk in Fig. 6B and 8B) was investigated by probing separate BN-PAGE Western blots with anti-gp120 and anti-gp41 cocktails (A) and by using biotinylated versions of Z13e1 and 2G12 for shifts and detecting with either the gp120/gp41 cocktail or strepavidin-alkaline phosphatase (streptavidin-AP) (B).