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Effects of enzyme digests on native PAGE shifts of E168K+N189A WT VLPs. Undigested E168K+N189A WT VLPs (A) and enzyme-digested E168K+N189A WT trimer VLPs (B) were incubated with various MAbs or sCD4 at 30 μg/ml each for 3 h at 37°C and then washed and resolved by a BN-PAGE Western blot. An ∼200-kDa band induced by the MAbs 4E10 and Z13e1 is indicated by an asterisk in panel B.
