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Effects of enzyme digests on native PAGE shifts of WT VLPs. Undigested WT VLPs (A), digested WT trimer VLPs (B), and SOS UNC VLPs (C) were incubated with various MAbs or sCD4 at 30 μg/ml for 3 h at 37°C and then washed and resolved by a BN-PAGE Western blot. An ∼200-kDa band induced by the MAbs 4E10 and Z13e1 is indicated by an asterisk in panel B. A high-molecular-weight species induced by the MAb CO11 is indicated by an arrow.
