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Transport of Qdot-labeled PR8 is primarily mediated by dermal dendritic cells. Qdot 655-labeled PR8 virus was used to coat MN and delivered into the skin in the ears of BALB/c mice. Auricular emigrants of explants were harvested from mice 10 min, 30 min, 2 h, 24 h, and 48 h postimmunization. Shown are representative flow cytometry analyses of Qdot-positive populations of total live cell emigrants, CD11c-negative cells, and dendritic cells (CD11c+).
