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. 2012 Mar 6;3(2):e00012-12. doi: 10.1128/mBio.00012-12

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Copyright © 2012 del Pilar Martin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 3 — Dendritic cells draining from skin and bearing PR8-Qdot antigen exhibit a mature phenotype. Results of flow cytometry analysis of DC emigrants from auricular explants after MN vaccination are shown. (A) Cells were evaluated for the expression of MHCI class II (MHC II). (B) Highly MHC II-positive DC (MHC II++) showing a distinct population of CD8⁺ CD205⁺ DC. (C) Plots of the latter showing the expression of maturation markers CD40 and CD86 and the prominent population positive for the PR8-Qdot label. The SD from two experiments with 3 to 5 mice are shown. (D) Fraction of MHC II++ DC negative for CD8 expression.