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(A) HPLC analyses from ethyl acetate-extracted broths from the Δtcs7/pFKBN strain (black line) and wild-type strain (gray line). The dotted lines indicate the identity of FK506 (circle) and FK520 (square). (B) Time course for FK506 production in R2YE medium. Triangles, FK506 concentration from the Δtcs7/pFKBN strain; circles, FK506 concentration from the Δtcs7 strain; squares, FK506 concentration from the WT/pFKBN strain; rhombuses, FK506 concentration from the wild-type strain.
