Table 1.
Strains, plasmids, and oligonucleotide primers
| Strain, plasmid, or primer | Description or sequence (5′ → 3′)a | Reference |
|---|---|---|
| Strains | ||
| C7258 | Wild type (El Tor, Ogawa) | Peru isolate, 1991 |
| C7258ΔlacZ | C7258 lacZ deletion mutant | 36 |
| AJB80 | C7258 ΔlacZ Δhns::Km | 36 |
| Plasmids | ||
| pTT3 | Transcription terminator rrnB T1T2 cloned in pUC19 | 35 |
| pKRZ1 | Plasmid containing promoterless lacZ gene | 33 |
| pTT3VpsA | 0.6-kb SphI-HindIII vpsA promoter inserted downstream of transcription terminator in pTT3 | This study |
| pVpsA -LacZ | 1.0-kb KpnI-HindIII terminator-vpsA promoter fragment inserted upstream of promoterless lacZ gene in pKRZ1 | This study |
| pTT3VpsL | 0.6-kb SphI-HindIII vpsL promoter fragment in pTT3 | This study |
| pVpsL -LacZ | 1.0-kb KpnI-HindIII terminator-vpsL fragment in pKRZ1 | This study |
| pTT3VpsR | span lang=DE>0.6-kb SphI-HindIII vpsR promoter fragment in pTT3 | This study |
| pVpsR -LacZ | 1.0-kb KpnI-HindIII terminator-vpsR fragment in pKRZ1 | This study |
| pTT3VpsT | 0.7-kb SphI-HindIII vpsT promoter fragment in pTT3 | This study |
| pVpsT -LacZ | 1.1-kb KpnI-HindIII terminator-vpsT fragment in pKRZ1 | This study |
| pBRHNS | Transcription terminator rrnB T1T2 and the hns locus were sequentially cloned in pBR322 | This study |
| Primers | ||
| HNS1 | CGCGGATCCGTGAGAAAAACAAGTGCCAC | |
| HNS2 | GATGCATGCTTCCTGAGCGAGAAGATAGC | |
| HNS-F31 | GATCGCATATGGTAATGTCGGAAATCACTAAG | |
| HNS-R32 | GATCGGCTCTTCAGCACAGAGCGAATTCTTCCAGAGA | |
| TcpA-F1 | GTTCATAATTTCGATCTCCACTCCG | |
| TcpA-R2 | GTTAACCACACAAAGTCACCTGCAA | |
| VC1922-F61 | TAGAAGGTTGACGAAACAAGCAATCA | |
| VC1922-R62 | GGTTCAACCACCATAGGTACGAGT | |
| VicH5 | GGGAAGCTTGTAATGTCGGAAATCA | |
| VicH396 | AGGAGATCTCAGAGCGAATTCTTCC | |
| VicH7 | GTTGCATGCATGTCGGAAATCACTA | |
| RpoS1020 | CTTCTGCAGTTACTTGTCGTCATCG | |
| RpsM-F51 | GCAACTGCGTGATGGTGTAGCTAA | |
| RpsM-R52 | GCTTGATCGGCTTACGCGGACC | |
| VpsA1 | GAAGCATGCGCGTAGTAGTGAATTTTTCC | |
| VpsA2 | GCGAAGCTTTACCTGATCTAACATCTCAC | |
| VpsAF | CCTTTATATCGCGCTTAAACTATATGT | |
| VpsAR | ATCCTCTTTAAAAAAGAAATTTCACAAAAT | |
| VpsL1 | GAAGCATGCGTAATGGTACGGGTTTCATA | |
| VpsL2 | GCGAAGCTTCCGTGATAGTTAGTAATGCG | |
| VpsLF1 | CTTTTCGATAAACGATACATATTATTTCT | |
| VpsLR1 | GCATGAAAATAAACTTTAGTTTACTTTTAT | |
| VpsR1 | GAAGCATGCGAGTCTGGTGATGATGAAGC | |
| VpsR2 | GCGAAGCTTAATCTGCTACTTGAGTACAG | |
| VpsRF | TGGGGCTTTTTTTATTTCCTCAAGAAA | |
| VpsRR | TCGATATTCCTTATTTTTATTGTTACGAT | |
| VpsT1 | GAAGCATGCGTTGGCATGTGGTTAAAGTG | |
| VpsT2 | GCGAAGCTTGCAAACATCAGAAAGCATTC | |
| VpsTF2 | AGGCTATGAATAAATTGAGTGGTAAGT | |
| VpsTR2 | GAAGACAAACGTTTAATCAAGCGGAA |
a
Restriction sites used for cloning are underlined.