Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype, markers, and further characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| One Shot TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74recA1araD139 Δ(ara leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| BL21 Star(DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3) | Invitrogen |
| DH5 α | supE44 ΔlacU169 ϕ80lacZΔM15 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
| EPI300-T1R | F−mcrA Δ(mrr-hsdRMS-mcrBC) (Strr) ϕ80d/acZΔM15 ΔlacX74recA1endA1araD139 Δ(ara-leu)7697 galUgalkλ−rpsLnupGtrfAtonAdhfr | Epicentre |
| C. defragrans 65Phen | Wild type | 17 |
| Plasmids | ||
| pCR2.1-TOPO | TOPO TA cloning vector; Amr KmrlacZα | Invitrogen |
| pCR4-TOPO | TOPO TA cloning vector; Amr KmrlacZα | Invitrogen |
| pBluescript SK(+/−) | Cloning vector; Amr KmrlacZα-ccdB | Stratagene |
| pCC1FOS | Cloning vector fosmid prepn, Chlr | Epicentre |
| pET42a(+) | Expression vector; Kmr | Novagen |
| pET42a(+)geoA | Expression vector; KmrgeoA | This study |
| pET42a(+)geoB | Expression vector; KmrgeoB | This study |