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. 2012 Apr;78(7):2128–2136. doi: 10.1128/AEM.07226-11

Table 2.

List of primers

Name Purpose Sequencea
GDHFd1 Nested PCR on N-terminal protein sequence ATGAACTGTAC(GC)CA(AG)GA(CT)TT
GDHFd2 AC(GC)CA(AG)GA(CT)TTCAT(CT)(AT)(GC)(GC)GC
GDHRev AC(GC)GG(CT)TC(GC)AC(GC)GC(GC)A(AG)(GC)GG
GDH-F1 PCR-mediated synthesis of DIG-labeled DNA probe ACGCAGGATTTCATCAGG
GDH-R1 TACGGGTTCGACGGCGAA
geoA_BglII_R Construction of expression vector pET42a(+)geoA AGATCTTCAGAACACCAGCACCGGCTTG
geoA_NdeI_F CATATGAACGACACCCAGGATTTCATTTCC
geoB_NdeI_F Construction of expression vector pET42a(+)geoB CATATGACCATCGATCACCAGCACATCTTC
geoB_SalI_R GTCGACCTAGCCAAGCAGGTACACTGAC
a

Restriction sites are underlined.