Table 2.
Name | Purpose | Sequencea |
---|---|---|
GDHFd1 | Nested PCR on N-terminal protein sequence | ATGAACTGTAC(GC)CA(AG)GA(CT)TT |
GDHFd2 | AC(GC)CA(AG)GA(CT)TTCAT(CT)(AT)(GC)(GC)GC | |
GDHRev | AC(GC)GG(CT)TC(GC)AC(GC)GC(GC)A(AG)(GC)GG | |
GDH-F1 | PCR-mediated synthesis of DIG-labeled DNA probe | ACGCAGGATTTCATCAGG |
GDH-R1 | TACGGGTTCGACGGCGAA | |
geoA_BglII_R | Construction of expression vector pET42a(+)geoA | AGATCTTCAGAACACCAGCACCGGCTTG |
geoA_NdeI_F | CATATGAACGACACCCAGGATTTCATTTCC | |
geoB_NdeI_F | Construction of expression vector pET42a(+)geoB | CATATGACCATCGATCACCAGCACATCTTC |
geoB_SalI_R | GTCGACCTAGCCAAGCAGGTACACTGAC |
Restriction sites are underlined.