Table 3.
Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Yield (%) | Purification (fold) |
---|---|---|---|---|---|
Cell extract | 3,160 | 175 | 0.056 | 100 | 1 |
1st DEAE fast flow | 269 | 111 | 0.41 | 71 | 12 |
Phenyl-Sepharose 6 fast flow | 46 | 101 | 2.19 | 58 | 69 |
2nd DEAE fast flow | 36 | 156 | 4.33 | 89 | 114 |