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Enzyme kinetics of native and recombinant GeDHa
| Substrate | Native GeDH |
Recombinant GeDH |
||||
|---|---|---|---|---|---|---|
| Km (μM) | Vmax (U mg−1) | kcat/Km(1 · 106 s−1 M−1) | Km (μM) | Vmax (U mg−1) | kcat/Km(1 · 106 s−1 M−1) | |
| Geraniol | 5 | 10 | 1.57 | 3.3 | 2.6 | 0.62 |
| Nerol | 45 | 18 | 0.31 | 23.2 | 9.1 | 0.31 |
| Citronellol | 86 | 11 | 0.10 | 57.5 | 3.7 | 0.05 |
| (S)-(−)-Perillyl alcohol | 7 | 18 | 2.02 | 4.4 | 19.9 | 3.55 |
| Cumic alcohol | 21 | 14 | 0.52 | 6.0 | 7.2 | 0.94 |
| Benzyl alcohol | 170 | 47 | 0.22 | 115.7 | 16.8 | 0.11 |
Values are apparent values.
