Table 6.
Citral conversion by soluble extracts of E. coli pET42a(+) and pET42a(+)geoBa
| Plasmid | Time (h) | Geranial (mM) | Neral (mM) | Geraniol (mM) | Nerol (mM) | Geranic acid (mM) |
|---|---|---|---|---|---|---|
| pET42a(+)geoB | 0 | 11.60 ± 0.15 | 7.20 ± 0.20 | 0 | 0 | 0 |
| 2 | 2.95 ± 0.15 | 3.25 ± 0.05 | 2.3 ± 0.1 | 1.4 ± 0.1 | 7.65 ± 0.15 | |
| 24 | 0.32 ± 0.08 | 0.25 ± 0.05 | 0 | 0 | 8.90 ± 1.10 | |
| pET42a(+) | 0 | 11.50 ± 0.10 | 6.75 ± 0.05 | 0 | 0 | 0 |
| 2 | 11.32 ± 0.28 | 6.95 ± 0.35 | 0 | 0 | 0 | |
| 24 | 8.15 ± 0.55 | 5.20 ± 0.40 | 0 | 0 | 0 |
a
Soluble extracts were assayed in duplicate with 0.5 mM NAD+ in a two-phase system with 20 mM citral in HMN. The organic phase serves as reservoir for the substrate as well as for the products and was analyzed by gas chromatography.