Table 1.
Summary of pH-controlled fermentations (10% xylose)a
Strain | Furfural concn (mM) | Furfural-dependent slow growth |
μ max (h−1) | Cell yield (g liter−1) | |
---|---|---|---|---|---|
μ (h−1) | Duration (h)b | ||||
LY180/pTrc99A | 0 | No slow phase | 0.23 ± 0.1 | 4.6 ± 0.1 | |
LY180/pTrc99A | 10 | <0.04 | >72 | <0.04 | 0.06 ± 0.01 |
LY180/pLOI4856 | 0 | No slow phase | 0.22 ± 0.01 | 4.5 ± 0.2 | |
LY180/pLOI4856 | 10 | 0.09 ± 0.01 | 22 ± 2 | 0.14 ± 0.01 | 3.6 ± 0.1 |
LY180 | 0 | No slow phase | 0.23 ± 0.01 | 3.6 ± 0.2 | |
LY180 | 8 | 0.09 ± 0.01 | 21 ± 1 | 0.16 ± 0.01 | 4.0 ± 0.1 |
LY180 | 10 | <0.04 | >72 | <0.04 | 0.08 ± 0.02 |
XW118c | 0 | No slow phase | 0.22 ± 0.03 | 3.1 ± 0.2 | |
XW118 | 8 | 0.01 ± 0.01 | 55 ± 2 | 0.14 ± 0.01 | 4.0 ± 0.1 |
XW118 | 10 | <0.04 | >72 | <0.04 | 0.04 ± 0.01 |
E. coliW | 0 | No slow phase | 0.23 ± 0.01 | 2.0 ± 0.1 | |
E. coli W | 8 | 0.18 ± 0.01 | 13 ± 1 | 0.19 ± 0.01 | 1.9 ± 0.1 |
E. coli W | 10 | 0.06 ± 0.02 | 21 ± 1 | 0.20 ± 0.02 | 2.0 ± 0.1 |
XW137d | 0 | No slow phase | 0.22 ± 0.01 | 2.0 ± 0.1 | |
XW137 | 8 | 0.11 ± 0.02 | 16 ± 3 | 0.19 ± 0.01 | 1.9 ± 0.1 |
XW137 | 10 | 0.02 ± 0.01 | 37 ± 2 | 0.12 ± 0.02 | 1.9 ± 0.1 |
E. coliW/pTrc99A | 0 | No slow phase | 0.23 ± 0.01 | 2.4 ± 0.2 | |
E. coliW/pTrc99A | 10 | 0.03 ± 0.01 | 26 ± 4 | 0.13 ± 0.01 | 2.0 ± 0.3 |
E. coli W/pLOI4856 | 0 | No slow phase | 0.22 ± 0.01 | 1.9 ± 0.1 | |
E. coli W/pLOI4856 | 10 | 0.07 ± 0.01 | 16 ± 2 | 0.18 ± 0.02 | 2.5 ± 0.3 |
Fermentations (n ≥ 2) were performed in AM1 medium with 100 g xylose liter−1 (27).
The duration of furfural-induced slow growth was estimated as the time of intersection using extrapolated rates.
XW118 (LY180 ucpA::kan).
XW137 (E. coli W ucpA::kan).