Fig 4.

Characterization of protein complex formation in cell extracts from QM9414 and the Δpkac1 mutant. Strains were grown in Mandels-Andreotti minimal medium with 1% (wt/vol) lactose as a carbon source. CKT057, comprising the cellulase-activating element (CAE), was used as a probe. Thirty micrograms of T. reesei cell extract was loaded. The most clearly altered protein complex in light is indicated by arrows. Competition experiments with 100-fold excesses of CKT057 (wild type) and CKT083 (oligonucleotide with deleted CAE) as indicated confirmed specific binding of complexes by abolishment of complex formation (CKT057; wild-type) or unaltered complex formation (CKT083; deleted binding site).