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. 2012 Apr;78(7):2168–2178. doi: 10.1128/AEM.06959-11

Table 1.

Oligonucleotides used in this study

Name Sequencea
qRT-cbh1F 5′-GAAGAACTGCTGTCTGGA-3′
qRT-cbh1R 5′-AATGGAGAGGCTGTTACC-3′
qRT-cbh2F 5′-CCAACGCCTTCTTCATCAC-3′
qRT-cbh2R 5′-CCAGACAAACGAATCCAGC-3′
qRT-ace1F 5′-GACAAGACGGATGTGTTCCAG-3′
qRT-ace1R 5′-GTTGAAGATGTCGGGCTGTG-3′
qRT-ace2F 5′-TCAACATCCTCCACCACCAGTC-3′
qRT-ace2R 5′-TGTCGGCGTACTCTCTCAGC-3′
qRT-xyr1F 5′-CTTCCTCCTCCTGCTCATCG-3′
qRT-xyr1R 5′-TCGTGTGCCCTAACAATGGTC-3′
pkac1F 5′-AACCACAGCGCCTTTCCAG-3′
pkac1R 5′-AATCGCGACCGGATCAGTC-3′
pkac1MF 5′-TTCCTCATCACCCTGTGG-3′
pkac1MR 5′-CGCCAATCTACCTAATACCG-3′
acy1D3F 5′-ATCTCGAGGCTCAGCTGTACATTACGAAC-3′
acy1D3R 5′-ATGGTACCTCTAGACGCGCTTCATCTTCTATG-3′
acy1D5R 5′-ATGGATCCGTGAGAGGCTCCTACACAC-3′
acy1D5F 5′-ATGCGGCCGCATTAAGTGACTTGTAACACCC-3′
acy1MF 5′-CTCTGGCAACGAGGAAGCAATG-3′
acy1MR 5′-GATCCCTCCCGACCATGATTG-3′
acy1R1 5′-CGACCCGGTGGATACATTTC-3′
acy1F1 5′-CGCCGCATGGACTACTTTG-3′
CKT057 5′-GATCGCTTCTTTATTGGGTAATATACAGCCAGGCGGGG-3′
CKT058 5′-GATCCCCCGCCTGGCTGTATATTACCCAATAAAGAAGC-3′
CKT083 5′-GATCGCTTCTTTAAAGGGTTTTATACAGCCAGGCGGGG-3′
CKT084 5′-GATCCCCCGCCTGGCTGTATAAAACCCTTTAAAGAAGC-3′
cbh2T_F1 5′-GGCTTGCTCGCTGACTGATAC-3′
cbh2T_F2 5′-GAGGGAGACGAGGTTGTGATG-3′
HPH_F1 5′-GATGTAGGAGGGCGTGGATATG-3′
HPH_R1 5′-GGGAGATGCAATAGGTCAGG-3′
a

Underlining indicates restriction sites introduced to facilitate cloning.