Localizations of the OTEMO-encoding gene and BamHI fragment subclones in an 11-kb region of P. putida ATCC 17453. The identified open reading frames are as follows, from left to right: putative DNA topoisomerase III (topo), OTEMO, 2,5-diketocamphane monooxygenase (DKCMO), a TetR-type regulator, lactone hydrolase, and a camR repressor that regulates the downstream camDCAB operon (camAB) (not shown) (5, 30).