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Comparison of the kinetic parameters of wild-type OTEMO and the Y53F mutanta
| OTEMO protein | Substrate or cosubstrate | Mean Km (μM) ± SD | Mean kcat (s−1) ± SD | kcat/Km (s−1 M−1) |
|---|---|---|---|---|
| Wild type | OT-CoA ester | 18 ± 4 | 4.8 ± 0.4 | 2.7 × 105 |
| NADPH | 3.6 ± 0.9 | NA | NA | |
| Y53F | OT-CoA ester | 10 ± 1 | 2.5 ± 0.1 | 2.5 × 105 |
| NADPH | 4.0 ± 1.0 | NA | NA |
OT, 2-oxo-Δ3-4,5,5-trimethylcyclopentenylacetic acid; NA, not applicable.
